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Electroporation as an efficient method of gene transfer
Author(s) -
Odani Noritaka,
Ito Kodai,
Nakamura Harukazu
Publication year - 2008
Publication title -
development, growth and differentiation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.864
H-Index - 66
eISSN - 1440-169X
pISSN - 0012-1592
DOI - 10.1111/j.1440-169x.2008.01037.x
Subject(s) - electroporation , biology , gene knockdown , transposable element , retrovirus , gene transfer , rna interference , microbiology and biotechnology , gene , embryo , genetics , computational biology , mutant , rna
Gene transfer by electroporation is an indispensable method for the study of developmental biology, especially for the study using chick embryos. Here we briefly review the principles of the method, and its application to chick embryos. Methods of transient misexpression and long‐term misexpression by retrovirus vector or transposon system, and knockdown by small interference RNA are reviewed.