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Retrovirus vector‐mediated gene transfer into the chick optic vesicle by in ovo electroporation
Author(s) -
Sakuta Hiraki,
Suzuki Ryoko,
Noda Masaharu
Publication year - 2008
Publication title -
development, growth and differentiation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.864
H-Index - 66
eISSN - 1440-169X
pISSN - 0012-1592
DOI - 10.1111/j.1440-169x.2008.01021.x
Subject(s) - in ovo , electroporation , biology , optic vesicle , retrovirus , gene transfer , microbiology and biotechnology , vector (molecular biology) , viral vector , transgene , gene , green fluorescent protein , embryo , genetics , recombinant dna , eye development , phenotype
Owing to its external position in the embryo, the chick eye has been used as a readily accessible model for studying the molecular mechanisms behind the patterning of the central nervous system. Although methods of genetic analysis have not been established as in the mouse, the chick is convenient for analyzing the functions of genes by in ov o electroporation of retroviral vectors. In this review, we describe the retroviral vector‐mediated transfer of genes into the chick optic vesicle by in ovo electroporation. A rapid, efficient, and sustained expression of transgenes is achieved by this approach.