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Drosophila NAT1 , a homolog of the vertebrate translational regulator NAT1/DAP5/p97 , is required for embryonic germband extension and metamorphosis
Author(s) -
Yoshikane Nami,
Nakamura Nao,
Ueda Ryu,
Ueno Naoto,
Yamanaka Shinya,
Nakamura Makoto
Publication year - 2007
Publication title -
development, growth and differentiation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.864
H-Index - 66
eISSN - 1440-169X
pISSN - 0012-1592
DOI - 10.1111/j.1440-169x.2007.00956.x
Subject(s) - biology , genetics , mutant , complementation , eif4g , regulator , transposable element , germline , gene , rna , ribosome
Translational regulation has been to shown to play major roles in the patterning of the early Drosophila embryo. The eIF4G family member NAT1/p97/DAP5 has been identified as a novel translational repressor. To genetically dissect the in vivo function of this unconventional eIF4G‐related translational regulator , Drosophila NAT1 (dNAT1 ) mutants were isolated using a reverse‐genetics approach. Four transposon insertion mutants and a deletion mutant affecting the dNAT1 locus were analyzed. Genetic complementation tests and germline rescue using a 12 kb dNAT1 genomic DNA fragment revealed these to be loss‐of‐function mutants. One P‐element insertion line, dNAT1 GS1. , shows severe embryonic lethality and abnormal germband extension. Abnormalities at metamorphosis were also found, including defective head eversion and salivary gland degeneration in the hypomorphic allele dNAT ex1 . A phenotypic analysis of dNAT1 mutants suggests that dNAT protein plays a specific rather than general role in translational regulation.