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Expression of Thy 1.2 surface antigen increases significantly during the murine mesenchymal stem cells cultivation period
Author(s) -
Eslaminejad Mohamadreza Baghaban,
Nadri Samad,
Hosseini Reza Hajji
Publication year - 2007
Publication title -
development, growth and differentiation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.864
H-Index - 66
eISSN - 1440-169X
pISSN - 0012-1592
DOI - 10.1111/j.1440-169x.2007.00932.x
Subject(s) - mesenchymal stem cell , antigen , cd34 , cd44 , bone marrow , flow cytometry , population , biology , microbiology and biotechnology , stem cell , doubling time , immunology , haematopoiesis , andrology , cell culture , cell , medicine , genetics , environmental health
This study sought to investigate the absence or expression of some surface antigens on murine mesenchymal stem cells (mMSCs) during the cultivation period of primary culture to passage 3 (equivalent to about 15 or 16 population doubling number). For this purpose, bone marrow cells from 6–8‐week‐old mice (either NMRI or Balb/c) were cultivated in 75‐cm 2 culture flask for three successive passages, in each of which the culture was examined for the expression of CD135, CD44, CD31, Thy1.2, CD11b, CD45, CD34, Vcam1, Sca‐1, and c‐Kit antigens, using flow cytometry. Passage‐3 cells from each strain can easily be differentiated into bone and fat, which was indicative of their mesenchymal nature. Our results demonstrated that for each given antigen, the percentages of the cells expressing that antigen had been changed by subcultures. The statistical analysis showed that nearly all differences between the passages were statistically significant. In this term, the expressional changes of Thy 1.2 seemed to be very significant in such a way that the expression increased to about half of the whole population in passage 3. In conclusion, it seems that this antigen could be considered as an enriching antigen for mMSCs population from bone marrow adherent cell culture.