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Distinct hemogenic potential of endothelial cells and CD41 + cells in mouse embryos
Author(s) -
Hashimoto Kazuaki,
Fujimoto Tetsuhiro,
Shimoda Yuri,
Huang Xin,
Sakamoto Hiroshi,
Ogawa Minetaro
Publication year - 2007
Publication title -
development, growth and differentiation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.864
H-Index - 66
eISSN - 1440-169X
pISSN - 0012-1592
DOI - 10.1111/j.1440-169x.2007.00925.x
Subject(s) - hemangioblast , biology , yolk sac , progenitor cell , microbiology and biotechnology , haematopoiesis , endothelial stem cell , lateral plate mesoderm , embryonic stem cell , stem cell , cd34 , mesoderm , embryo , immunology , genetics , gene , in vitro
Definitive hematopoietic progenitor cells have been thought to develop from the vascular endothelium located in the aorta‐gonad‐mesonephros region of the mouse embryo. However, several recent findings have suggested that most hematopoietic progenitors are derived from non‐endothelial precursor cells expressing CD41. We characterized two distinct precursor populations of definitive hematopoietic cell lineages, vascular endothelial (VE)‐cadherin + CD41 − CD45 − endothelial cells and CD41 + CD45 − non‐endothelial progenitors, both of which are derived from lateral mesoderm. VE‐cadherin + endothelial cells obtained from cultures of differentiating embryonic stem cells possessed hematopoietic potential encompassing erythroid, myeloid and B lymphoid lineages, whereas CD41 + progenitors lacked the B lymphopoietic potential. VE‐cadherin + endothelial cells in the lower trunk of the embryo proper showed a significant potential for initiating B lymphopoiesis in cultures, while endothelial cells in the yolk sac appeared to have a bias for myeloerythropoietic differentiation. CD41 + progenitors isolated from yolk sac and embryo proper were capable of generating multiple hematopoietic lineages, although mast cell precursors were exclusively enriched in CD41 + progenitors in the yolk sac. These results suggest that hemogenic endothelial cells and CD41 + progenitors possess distinct hematopoietic potential depending on the tissues in which they reside.