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Mi‐2 chromatin remodeling factor functions in sensory organ development through proneural gene repression in Drosophila
Author(s) -
Yamasaki Yasutoyo,
Nishida Yasuyoshi
Publication year - 2006
Publication title -
development, growth and differentiation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.864
H-Index - 66
eISSN - 1440-169X
pISSN - 0012-1592
DOI - 10.1111/j.1440-169x.2006.00880.x
Subject(s) - biology , imaginal disc , chromatin remodeling , chromatin , histone , nucleosome , chromatin immunoprecipitation , microbiology and biotechnology , polytene chromosome , locus (genetics) , acetylation , transcription factor , swi/snf , mutant , drosophila melanogaster , genetics , gene , gene expression , promoter
Mi‐2, the central component of the nucleosome remodeling and histone deacetylation (NuRD) complex, is known as an SNF2‐type ATP‐dependent nucleosome remodeling factor. No morphological mutant phenotype of Drosophila Mi‐2 ( dMi‐2 ) had been reported previously; however, we found that rare escapers develop into adult flies showing an extra bristle phenotype. The dMi‐2 enhanced the phenotype of ac Hw49c , which is a dominant gain‐of‐function allele of achaete ( ac ) and produces extra bristles. Consistent with these observations, the ac ‐expressing proneural clusters were expanded, and extra sensory organ precursors (SOP) were formed in the dMi‐2 mutant wing discs. Immunostaining of polytene chromosomes showed that dMi‐2 binds to the ac locus, and dMi‐2 and acetylated hisotones distribute on polytene chromosomes in a mutually exclusive manner. The chromatin immunoprecipitation assay of the wing imaginal disc also demonstrated a binding of dMi‐2 on the ac locus. These results suggest that the Drosophila Mi‐2/NuRD complex functions in neuronal differentiation through the repression of proneural gene expression by chromatin remodeling and histone deacetylation.