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Generation of new human embryonic stem cell lines with diploid and triploid karyotypes
Author(s) -
Baharvand Hossein,
Ashtiani Saeid Kazemi,
Taee Adeleh,
Massumi Mohammad,
Valojerdi Mojtaba R.,
Yazdi Poopak E.,
Moradi Shabnam Z.,
Farrokhi Ali
Publication year - 2006
Publication title -
development, growth and differentiation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.864
H-Index - 66
eISSN - 1440-169X
pISSN - 0012-1592
DOI - 10.1111/j.1440-169x.2006.00851.x
Subject(s) - homeobox protein nanog , sox2 , biology , embryonic stem cell , kosr , induced pluripotent stem cell , cell culture , stem cell , microbiology and biotechnology , karyotype , rex1 , genetics , chromosome , gene
Human pluripotent embryonic stem cells (hESC) have great promise for research into human developmental biology and the development of cell therapies for the treatment of diseases. To meet the increased demand for characterized hESC lines, we present the derivation and characterization of five hESC lines on mouse embryonic fibroblast cells. Our stem cell lines are characterized by morphology, long‐term expansion, and expression profiles of a number of specific markers, including TRA‐1‐60, TRA‐1‐81, alkaline phosphatase, connexin 43, OCT‐4, NANOG, CXCR4, NODAL, LEFTY2, THY‐1, TDGF1, PAX6, FOXD3, SOX2, EPHA2, FGF4, TAL1, AC133 and REX‐1 . The pluripotency of the cell line was confirmed by spontaneous differentiation under in vitro conditions. Whereas all of the cell lines expressed all the characteristics of undifferentiated pluripotent hESC, two of the cell lines carried a triploid karyotype.