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Runx3 controls growth and differentiation of gastric epithelial cells in mammals
Author(s) -
Fukamachi Hiroshi
Publication year - 2006
Publication title -
development, growth and differentiation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.864
H-Index - 66
eISSN - 1440-169X
pISSN - 0012-1592
DOI - 10.1111/j.1440-169x.2006.00832.x
Subject(s) - microbiology and biotechnology , biology , evolutionary biology
Runx3 is a transcription factor expressed by gastric epithelial cells. In the Runx3 −/– mouse, gastric epithelia exhibited hyperplasia, and epithelial apoptosis was suppressed. By analyzing growth of the epithelial cells in primary culture, we found that Runx3 −/– gastric epithelial cells are less sensitive to the growth‐inhibitory and apoptosis‐inducing activities of TGF‐β, suggesting that Runx3 is a major growth regulator of gastric epithelial cells by regulating their response to TGF‐β. We also found that Runx3 plays an important role in the control of gastric epithelial differentiation. When subcutaneously implanted into nude mice, Runx3 −/– gastric epithelial cells formed tumors in which some cells differentiated into intestinal‐type cells. Clonal analysis showed that gastric epithelial cells transdifferentiate into intestinal‐type cells in the tumor. Considering that gastric epithelial differentiation is very stable, and that intestinal‐type cells never differentiate in the mouse stomach, it is remarkable that gastric epithelial cells transdifferentiate into intestinal‐type cells. We conclude that Runx3 is deeply involved in the control of both growth and differentiation of gastric epithelial cells. The role of Runx3 in the specification of gastric epithelial cells is discussed.