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TGF‐β3 induces ectopic mineralization in fetal mouse dental pulp during tooth germ development
Author(s) -
Huojia Muhetaer,
Muraoka Noriko,
Yoshizaki Keigo,
Fukumoto Satoshi,
Nakashima Misako,
Akamine Akifumi,
aka Kazuaki,
Ohishi Masamichi
Publication year - 2005
Publication title -
development, growth and differentiation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.864
H-Index - 66
eISSN - 1440-169X
pISSN - 0012-1592
DOI - 10.1111/j.1440-169x.2005.00790.x
Subject(s) - dentin sialophosphoprotein , dmp1 , odontoblast , osteopontin , calvaria , pulp (tooth) , chemistry , osteocalcin , extracellular matrix , dental pulp stem cells , dentinogenesis , dental papilla , microbiology and biotechnology , dentin , dentistry , biology , stem cell , endocrinology , alkaline phosphatase , medicine , viral matrix protein , biochemistry , in vitro , gene , enzyme
Several members of the transforming growth factor (TGF)‐β superfamily are expressed in developing teeth from the initiation stage through adulthood. Of those, TGF‐β1 regulates odontoblast differentiation and dentin extracellular matrix synthesis. However, the molecular mechanism of TGF‐β3 in dental pulp cells is not clearly understood. In the present study, beads soaked with human recombinant TGF‐β3 induced ectopic mineralization in dental pulp from fetal mouse tooth germ samples, which increased in a dose‐dependent manner. Further, TGF‐β3 promoted mRNA expression, and increased protein levels of osteocalcin (OCN) and type I collagen (COL I) in dental pulp cells. We also observed that the expression of dentin sialophosphoprotein and dentin matrix protein 1 was induced by TGF‐β3 in primary cultured dental pulp cells, however, not in calvaria osteoblasts, whereas OCN, osteopontin and osteonectin expression was increased after treatment with TGF‐β3 in both dental pulp cells and calvaria osteoblasts. Dentin sialoprotein was also partially detected in the vicinity of TGF‐β3 soaked beads in vivo . These results indicate for the first time that TGF‐β3 induces ectopic mineralization through upregulation of OCN and COL I expression in dental pulp cells, and may regulate the differentiation of dental pulp stem cells to odontoblasts.