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Cloning and characterization of the tomato karyopherin α1 gene promoter
Author(s) -
Mizrachy Liat,
Dabush David,
Levy Yael,
Aloni Roni,
Altman Arie,
Gafni Yedidya
Publication year - 2004
Publication title -
development, growth and differentiation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.864
H-Index - 66
eISSN - 1440-169X
pISSN - 0012-1592
DOI - 10.1111/j.1440-169x.2004.00766.x
Subject(s) - karyopherin , biology , lycopersicon , gene , cloning (programming) , clone (java method) , microbiology and biotechnology , promoter , gene expression , transgene , beta glucuronidase , reporter gene , glucuronidase , gus reporter system , genetics , botany , nuclear transport , cell nucleus , computer science , programming language
The karyopherin α1 ( LeKAPα 1) gene of tomato ( Lycopersicon esculentum ) encodes a receptor involved in nuclear import. To analyze the expression pattern of this gene, a genomic clone containing its upstream region was isolated and sequenced. To study the promoter functionality, a 2170 bp fragment (LM1), was fused to glucuronidase (GUS) and introduced into petunia cells by particle bombardment. For further characterization of the promoter, one inverse and three deletion constructs were studied in cell suspension. To follow its expression in tobacco leaves, transgenic plants expressing GUS under the control of the LM1 promoter were made. Expression of LM1–GUS was largely restricted to actively growing leaf regions, suggesting possible involvement of active cell division and plant growth regulators in LeKAPα 1 expression.