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Minos transposon causes germline transgenesis of the ascidian Ciona savignyi
Author(s) -
Matsuoka Terumi,
Awazu Satoko,
Satoh Nori,
Sasakura Yasunori
Publication year - 2004
Publication title -
development, growth and differentiation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.864
H-Index - 66
eISSN - 1440-169X
pISSN - 0012-1592
DOI - 10.1111/j.1440-169x.2004.00742.x
Subject(s) - ciona intestinalis , ciona , biology , transposable element , transgenesis , insertional mutagenesis , genetics , germline , mutagenesis , genome , gene , mutation , reproductive biology , embryogenesis
An ascidian, Ciona savignyi , is regarded as a good experimental animal for genetics because of its small and compact genome for which a draft sequence is available, its short generation time and its interesting phylogenic position. ENU‐based mutagenesis has been carried out using this animal. However, insertional mutagenesis using transposable elements (transposons) has not yet been introduced. Recently, one of the Tc1/ mariner superfamily transposons, Minos , was demonstrated to cause germline transgenesis in the related species Ciona intestinalis . In this report, we show that Minos has the ability to transpose from DNA to DNA in Ciona savignyi in transposition assays. Although the activity was slightly weaker than in Ciona intestinalis , Minos still caused germline transgenesis in Ciona savignyi . In addition, one insertion seemed to have caused an enhancer trapping. These results indicate that Minos provides a potential tool for transgenic techniques such as insertional mutagenesis in Ciona savignyi .