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Hoxa‐11 and Hoxa‐13 are involved in repression of MyoD during limb muscle development
Author(s) -
Yamamoto Masakazu,
Kuroiwa Atsushi
Publication year - 2003
Publication title -
development, growth and differentiation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.864
H-Index - 66
eISSN - 1440-169X
pISSN - 0012-1592
DOI - 10.1111/j.1440-169x.2003.00715.x
Subject(s) - myod , myogenesis , c2c12 , myocyte , myod protein , ectopic expression , psychological repression , biology , microbiology and biotechnology , gene expression , gene , genetics
Under the influence of the limb mesenchyme, Hoxa‐11 is expressed in migrating and proliferating premyoblasts in the limb field and Hoxa‐13 is induced in subdomains of congregated limb muscle masses. To evaluate the roles of Hoxa‐11 and Hoxa‐13 in myogenesis of the limb, we performed electroporation in ovo to force expression of these Hox genes in limb muscle precursors. In the presence of ectopic Hoxa‐11, expression of MyoD was blocked transiently. In C2C12 myoblasts, transfection of Hoxa‐11 also repressed the expression of endogenous MyoD . Forced expression of Hoxa‐13 resulted in more pronounced repression of MyoD in both limb and C2C12 myoblasts. In contrast, targeted disruption of Hoxa‐13 gave rise to enhanced expression of MyoD in the flexor carpi radialis muscle, a forearm muscle that normally expressed Hoxa‐13 . These results suggest that Hoxa‐11 and Hoxa‐13 are involved in the negative regulation of MyoD expression in limb muscle precursors.