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A simplified and efficient method for in situ hybridization to whole Drosophila embryos, using electrophoresis for removing non‐hybridized probes
Author(s) -
Kobayashi Satoru,
Saito Hiromitsu,
Okada Masukichi
Publication year - 1994
Publication title -
development, growth and differentiation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.864
H-Index - 66
eISSN - 1440-169X
pISSN - 0012-1592
DOI - 10.1111/j.1440-169x.1994.00629.x
Subject(s) - in situ hybridization , embryo , electrophoresis , in situ , drosophila (subgenus) , signal (programming language) , biology , biological system , microbiology and biotechnology , chemistry , chromatography , genetics , computer science , messenger rna , gene , organic chemistry , programming language
We report a simplified and reliable method for non‐radioactive in situ hybridization to whole Drosophila embryos. In the previous method (Tautz and Pfeifle, 1989) the post‐hybridization wash, or the procedure for washing non‐hybridized probe away from embryos depends simply on diffusion. We modified the method with application of electrophoresis to the wash. After hybridized with RNA probe, embryos were transferred to a small well where an electric charge was given to drive non‐hybridized probe away from the embryos. This procedure enables us to acquire a much higher signal‐to‐noise ratio than that obtained from a conventional method. Furthermore, this is a time‐saving method. We propose a term “electro‐wash” for this procedure.