Premium
F9 Cells Can be Differentiated toward Two Distinct, Mutually Exclusive Pathways by Retinoic Acid and Sodium Butyrate
Author(s) -
Kosaka Mitsuko,
Takeda Masashi,
Matsumoto Keishi,
Nishimune Yoshitake
Publication year - 1994
Publication title -
development, growth and differentiation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.864
H-Index - 66
eISSN - 1440-169X
pISSN - 0012-1592
DOI - 10.1111/j.1440-169x.1994.00223.x
Subject(s) - sodium butyrate , retinoic acid , butyrate , phenotype , cellular differentiation , tretinoin , embryonal carcinoma , microbiology and biotechnology , chemistry , biology , cell culture , biochemistry , genetics , gene , fermentation
Both retinoic acid (RA) and sodium butyrate (NaB) induce differentiation in embryonal carcinoma F9 cells. Phenotypic changes caused by RA are irreversible, whereas those of NaB are rapid and reversible. In this study, we investigated the effects of combinations of these two agents on F9 cell differentiation and showed that RA had no effect on the cells induced to differentiate with NaB and vice versa. Thus, F9 cells are induced to differentiate along two distinct pathways which are mutually exclusive.