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Does ADP‐Ribosylation of Proteins in Nuclei Contribute to Ectoderm Cell Differentiation in Sea Urchin Embryos?
Author(s) -
Kamata Yasuyuki,
Fujiwara Akiko,
Furuya Shigehisa,
Yasumasu Ikuo
Publication year - 1993
Publication title -
development, growth and differentiation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.864
H-Index - 66
eISSN - 1440-169X
pISSN - 0012-1592
DOI - 10.1111/j.1440-169x.1993.00089.x
Subject(s) - ectoderm , biology , gastrulation , sea urchin , microbiology and biotechnology , hatching , embryo , endoderm , embryogenesis , morphogenesis , cellular differentiation , genetics , ecology , gene
In nuclei of sea urchin embryos, marked increase in ADP‐ribosyltransferase activity followed by its decrease occurrs in the pre‐hatching and post‐hatching periods with peaks of activity at the morula and gastrula stages. Increase in its activity was blocked by cycloheximide in the pre‐ and post‐hatching periods and by actinomycin D only in the post‐hatching period. Embryo wall cells (ectoderm cells) isolated from gastrulae exhibited markedly higher activity of this enzyme than archenteron cells and mesenchyme cells. Probably, the increase in the activity of this enzyme in the post‐hatching period results from expression of the gene for this enzyme mainly in ectoderm cells. In the post‐hatching period, the activity increased more in animalized embryos than in normal ones, and increased little in vegetalized embryos. 3‐Aminobenzamide (3‐ABA), as well as luminol and nicotinamide, inhibited formation of ectoderm structures more than that of endoderm structures, such as the archenteron, in normal and animalized embryos, but had no appreciable effect on morphogenesis in vegetalized embryos. The reaction catalyzed by ADP‐ribosyltransferase probably contributes to ectoderm cell differentiation. Treatment of embryos with 3‐ABA in the pre‐hatching period had little inhibitory effect on the morphogenesis in the post‐hatching period, though it caused death of many embryos.