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Effect of Microtubular Poisons on Cleavage Furrow Formation and Induction of Furrow‐like Dent in Amphibian Eggs
Author(s) -
Sawai Tsuyoshi
Publication year - 1992
Publication title -
development, growth and differentiation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.864
H-Index - 66
eISSN - 1440-169X
pISSN - 0012-1592
DOI - 10.1111/j.1440-169x.1992.tb00035.x
Subject(s) - cleavage furrow , cleavage (geology) , nocodazole , blastomere , cytokinesis , microbiology and biotechnology , chemistry , anatomy , biology , embryo , cell , cell division , biochemistry , embryogenesis , paleontology , cytoskeleton , fracture (geology)
The effects of the microtubular poisons colchicine, vinblastine and nocodazole, on cleavage furrow formation and induction of furrow‐like dents in eggs of the newt, Cynops pyrrhogaster , were examined. Solutions of the poisons were injected beneath the cortex around the small initial furrow, or around the advancing tip of the furrow of eggs during the first cleavage. This resulted in prompt block of the progress of the furrow at the injection site, and subsequent total regression of the furrow or incomplete cleavage. The ability of the cortex of a cleavage‐arrested blastomere to form a furrow‐like dent was tested by inhibiting furrow formation of one blastomere of two‐cell embryos by injection of the microtubular poisons, and then transplantation of the blastomere under the cortex of the animal half with furrow‐inducing cytoplasm (FIC) taken from normally cleaving eggs. No dent was formed. Moreover, FIC from eggs treated with a poison had no ability to induce a dent on the surface of normally cleaving eggs. These results show that microtubule structures are directly involved in formation of a cleavage furrow.