Premium
Changes in Electrophoretic Patterns of Oocyte Proteins during Oocyte Maturation in Oryzias latipes
Author(s) -
Iwamatsu Takashi,
Takahashi Susumu Y.,
Ohishi Masamichi,
Yokochi Takashi,
Maeda Hiroko
Publication year - 1992
Publication title -
development, growth and differentiation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.864
H-Index - 66
eISSN - 1440-169X
pISSN - 0012-1592
DOI - 10.1111/j.1440-169x.1992.tb00006.x
Subject(s) - germinal vesicle , oocyte , oryzias , biology , microbiology and biotechnology , chemistry , andrology , biochemistry , embryo , gene , medicine
Changes in the two‐dimensional SDS‐electrophoretic patterns of extracts of maturing denuded oocytes of the medaka ( Oryzias latipes ) were surveyed. In oocytes without follicular constituents several proteins became detectable in the area between the acidic and slightly basic proteins on the two‐dimensional electrophoretograms, while a few of the protein spots disappeared during the process of oocyte maturation. The former proteins were detected also in oocytes that were induced to mature in vivo without breakdown of the germinal vesicle. Several proteins newly observed in extracts of post‐vitellogenic oocytes during maturation after breakdown of the germinal vesicle were also identified by two‐dimensional electrophoresis. Of several proteins that exhibited noticeable changes in maturing oocytes, only one spot incorporated 14 C‐labeled amino acid during maturation, suggesting that post‐translational modification of many proteins occurred during oocyte maturation.