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Pathogenesis in Transgenic Mice Expressing Bovine Cellular Retinoic Acid‐Binding Protein
Author(s) -
Wei LiNa,
Lee ChihHao,
Chang ShuLing,
Chu YaShu
Publication year - 1992
Publication title -
development, growth and differentiation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.864
H-Index - 66
eISSN - 1440-169X
pISSN - 0012-1592
DOI - 10.1111/j.1440-169x.1992.00479.x
Subject(s) - retinoic acid , genetically modified mouse , pathogenesis , transgene , microbiology and biotechnology , biology , immunology , gene , biochemistry
Transgenic mice with ectopic expression of bovine CRABP under the control of the human metallotheionein IIA promoter have shown a variety of pathological consequences. Expression of the transgene has been detected in most of the tissues examined, including heart, lung, liver, spleen, kidney, intestine, testis, and ovary, except pancreas. Two independent lines have been able to produce normal non‐transgenic F 1 animals of both sexes but only female transgenic progenies. All of these F 1 female transgenic animals derived from both lines are sterile, and the ovaries from these animals appear to be significantly smaller as compared to their non‐transgenic littermates. Histopathological examinations have shown no maturing follicles in these transgenic ovaries in which abnormal cells have been observed. Another independent line has generated transgenic F 1 animals which have been growing retardly. These animals all have small spleen and liver and have become very sick at the age of 4 to 5 weeks. Histopathological examinations on these transgenic progenies have shown hepatocytes to be reduced in the cytoplasmic portion in which glycogen is highly depleted. The spleen is poorly developed as no well organized germinal centers can be observed in the spleen sections of these transgenic animals.