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Differential Effects of the Egg Jelly Molecules FSG and SAP‐I on Elevation of Intracellular Ca 2+ and pH in Sea Urchin Spermatozoa
Author(s) -
Hoshino Katsuaki,
Shimizu Takeshi,
Sendai Yutaka,
Harumi Tatsuo,
Suzuki Norio
Publication year - 1992
Publication title -
development, growth and differentiation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.864
H-Index - 66
eISSN - 1440-169X
pISSN - 0012-1592
DOI - 10.1111/j.1440-169x.1992.00403.x
Subject(s) - sea urchin , chemistry , hemicentrotus , intracellular ph , intracellular , channel blocker , acrosome reaction , sperm , extracellular , biophysics , biochemistry , biology , calcium , botany , microbiology and biotechnology , in vitro , organic chemistry
We examined the effects of two egg jelly components, a fucose sulfate glycoconjugate (FSG) and sperm‐activating peptide I (SAP‐I: Gly‐Phe‐Asp‐Leu‐Asn‐Gly‐Gly‐Gly‐Val‐Gly), on the intracellular pH (pH i ) and Ca 2+ ([Ca 2+ ] i ) of spermatozoa of the sea urchin Hemicentrotus pulcherrimus . FSG and/or SAP‐I induced elevations of [Ca 2+ ]; and pH i in the spermatozoa at pH 8.0. At pH 8.0, a second addition of FSG did not induced further elevation of the [Ca 2+ ] i or pH i of spermatozoa treated with FSG, but addition or FSG after SAP‐I or of SAP‐I after FSG induced further increases of [Ca 2+ ] i and pH i , At pH 6.6, FSG and/or SAP‐I did not induce significant elevation of the [Ca 2+ ] i , although SAP‐I elevated the pH i , its half‐maximal effective concentration being 10 to 100 pM. At pH 8.0, tetraethyl‐ammonium, a voltage‐sensitive K + ‐channel blocker, inhibited induction of the acrosome reaction and elevations of [Ca 2+ ] i and pH i by FSG, but did not affect those by SAP‐I. These results suggest that FSG and SAP‐I activate different Ca 2+ and H + transport systems.