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Immunoelectophoretic Identification of Jelly Coat Ligands Bound by the Cortical Granule Lectin from Xenopus laevis Eggs
Author(s) -
Birr Cynthia A.,
Hedrick Jerry L.
Publication year - 1992
Publication title -
development, growth and differentiation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.864
H-Index - 66
eISSN - 1440-169X
pISSN - 0012-1592
DOI - 10.1111/j.1440-169x.1992.00091.x
Subject(s) - xenopus , lectin , coat , chemistry , c type lectin , microbiology and biotechnology , biology , biochemistry , ecology , gene
The formation of the fertilization layer in the Xenopus laevis egg fertilization envelope involves a lectin‐ligand interaction and establishes a block to polyspermy in the extracellular matrix of the egg. The cortical granule lectin participating in the formation of the fertilization layer has been isolated but its ligand has not. We identified three jelly coat ligands bound by the cortical granule lectin using immunoelectrophoretic analyses. Two antigens were detected with anti‐jelly serum and a third was identified using anti‐envelope serum. All three antigenic ligands were associated with the innermost jelly coat layer, J 1 , and two of the three antigenic ligands contained sulfate. One or more of these jelly coat ligands may function in establishing a block to polyspermy at fertilization in Xenopus laevis .