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Partial Characterization of Macromolecular Components in Fetal Bovine Serum Required for Development of Mouse Blastocysts Cultured in vitro
Author(s) -
Tachi Chikashi
Publication year - 1992
Publication title -
development, growth and differentiation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.864
H-Index - 66
eISSN - 1440-169X
pISSN - 0012-1592
DOI - 10.1111/j.1440-169x.1992.00069.x
Subject(s) - in vitro , trophoblast , andrology , biology , embryo , blastocyst , embryogenesis , microbiology and biotechnology , inner cell mass , fetus , embryo culture , immunology , pregnancy , placenta , genetics , medicine
Elucidation of the mechanisms underlying implantation of blastocysts in eutherian mammals have been severely hampered by the lack of a suitable system for culture of blastocysts in vitro . Successful culture methods for mouse peri‐implantation embryos in vitro have been described by Hsu (1971, 1973, 1978, 1990), Chen and Hsu (1982) and Naruse et al. (1985), but these methods are too complex for routine experimental purposes. We attempted, therefore, to establish a standard culture method suitable for quantitative analysis of early embryogenesis in the mouse. Our system allows the development of peri‐implantation blastocysts from the time of shedding of the zona to formation of the proamniotic cavity under well defined conditions. Using this system, the macromolecular components in the sera required for the development of periimplantation mouse blastocysts in vitro were partially characterized. Results indicated that substances with molecular weight (MW) of 30 × 10 3 to 100 × 10 3 in the serum are capable of inducing the early phase of the trophoblast spreading. Furthermore, serum factors above MW 100 × 10 3 were found to be essential for the successful differentiation and/or development of ICM and ectoplacental cones.