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Mesoderm Induction in Pleurodeles waltl : Distribution of Fibronectin and Chondroitin Sulfate in Induced Explants
Author(s) -
LøvtrupRein H.,
Umbhauer M.,
Riou J.F.,
Boucaut J.C.
Publication year - 1992
Publication title -
development, growth and differentiation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.864
H-Index - 66
eISSN - 1440-169X
pISSN - 0012-1592
DOI - 10.1111/j.1440-169x.1992.00061.x
Subject(s) - pleurodeles , mesoderm , biology , gastrulation , microbiology and biotechnology , xenopus , fibronectin , extracellular matrix , embryo , embryogenesis , embryonic stem cell , biochemistry , amphibian , ecology , gene
In Pleurodeles , cell‐matrix interactions play a major role in promoting active mesodermal cell migration during gastrulation. It was therefore important to determine whether the expression of define matrix molecules may be dependent on mesoderm induction. Results from induction experiments done with XTC cell line‐conditioned medium show that mesoderm tissues induced in animal cap explants of Pleurodeles are identical to those from Xenopus . However, we also show that dorsally‐induced explants in Pleurodeles elongate to a lesser degree than in Xenopus . This observation agrees well with the differences observed in the role of ECM in Pleurodeles and Xenopus gastrulation, respectively. Additional immunostaining studies demonstrate that the induction of mesodermal tissues is associated with the expression of chondroitin sulfate whereas fibronectin fibrils are already assembled in uninduced animal caps. These results suggest that mesoderm cell‐matrix interactions in early amphibian embryo may be under the control of mesoderm induction.