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A Novel Monoclonal Antibody disrupting Cell Type Specific Substratum Adhesion of Frog ( Xenopus laevis ) Epithelial Cells and Endothelial Cells
Author(s) -
Nomura Kazuya,
Tajima Tatsuya,
Nomura Hajime,
Tsuno Kiyomi,
Fujimura Yasuo,
Nakajo Nobushige,
Yamana Kiyotaka
Publication year - 1991
Publication title -
development, growth and differentiation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.864
H-Index - 66
eISSN - 1440-169X
pISSN - 0012-1592
DOI - 10.1111/j.1440-169x.1991.00639.x
Subject(s) - xenopus , monoclonal antibody , epitope , fibronectin , biology , microbiology and biotechnology , antibody , antigen , cell adhesion , adhesion , cell type , cell , african clawed frog , chemistry , immunology , extracellular matrix , biochemistry , organic chemistry , gene
We isolated a mouse monoclonal antibody (FAD‐II) that disrupts cell‐substratum adhesion of amphibian ( Xenopus laevis ) epithelial cells and endothelial cells. The effect of the antibody was cell‐type specific, and the antibody had no effect on fibroblastic cells while fibronectin peptide blocked cell‐substratum adhesion of all the cell types examined. In developing frog embryos, the epitopes recognized by the antibody were detected in pronephrotic ducts and in other tissue cells of embryos (from stage 33/34 afterwards). In adult tissues, the antibody mainly recognized antigens in extracelluar matrices. The antigens recognized by the antibody seems to be novel glycoepitopes in frog cells.