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Developmental Changes in the Bombyxin‐and Insulin‐like Immunoreactive Neurosecretory System in the Wax Moth, Galleria mellonella
Author(s) -
Žitňn Dušn,
Sehnal František,
Mizoguchi Akira,
Ishizaki Hironori,
Nagasawa Hiromichi,
Suzuki Akinori
Publication year - 1990
Publication title -
development, growth and differentiation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.864
H-Index - 66
eISSN - 1440-169X
pISSN - 0012-1592
DOI - 10.1111/j.1440-169x.1990.00637.x
Subject(s) - ecdysis , galleria mellonella , medicine , endocrinology , corpus allatum , biology , stimulation , period (music) , prothoracic gland , instar , larva , hormone , ecdysone , ecology , juvenile hormone , biochemistry , virulence , gene , physics , acoustics
Four medial neurosecretory cells (MNC) and 4 lateral neurosecretory cells (LNC) in each brain hemisphere, and one pair of cells in each thoracic ganglion (TG) of Galleria larva react with antibodies against bombyxin and insulin. Material secreted from the MNC and LNC is released mainly in the corpora allata, and that from the TG through the ventral median nerves. Intrinsic secretory cells of the corpora cardiaca (CC) also contain bombyxin‐like, but not insulin‐like material. The immunoreactivities all disappear during molts and reappear with resumption of feeding. In the MNC and TG they reappear for less than a day, but in cells of the CC immunoreactivity reappears for the whole feeding period. Before pupation, the LNC become temporarily immunopositive towards the end of feeding period, and the MNC and TG during the wandering period, i.e. at the time of prothoracic gland stimulation. Immunoreactivity disappears during the pupal molt. In pupae it is present in the 4 pairs of MNC and 1–2 pairs of LNC 12–48 hr after ecdysis, and in cells of the CC from 12 hr after ecdysis until the end of the pupal instar. In adult, immunoreactivity is restricted to 2 pairs of the LNC and to CC cells.