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Immunohistochemical Localization of Prothoracicotropic Hormone‐Producing Neurosecretory Cells in the Brain of Bombyx mori
Author(s) -
Mizoguchi Akira,
Oka Tadanori,
Kataoka Hiroshi,
Nagasawa Hiromichi,
Suzuki Akinori,
Ishizaki Hironori
Publication year - 1990
Publication title -
development, growth and differentiation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.864
H-Index - 66
eISSN - 1440-169X
pISSN - 0012-1592
DOI - 10.1111/j.1440-169x.1990.00591.x
Subject(s) - bombyx mori , immunohistochemistry , bombyx , corpus allatum , axon , monoclonal antibody , endocrinology , medicine , biology , antibody , hormone , chemistry , microbiology and biotechnology , juvenile hormone , biochemistry , immunology , gene
A monoclonal antibody that recognized the Bombyx prothoracicotropic hormone (PTTH) was produced by immunizing mice with a synthetic pentadecapeptide corresponding to the amino‐terminal portion of Bombyx PTTH. The antibody recognized both intact and reduced forms of PTTH. Immunohistochemistry with this antibody has demonstrated that PTTH is produced by two pairs of dorso‐lateral neurosecretory cells of the brain and transported to the corpora allata by axons running through the contralateral hemisphere of the brain. Immunoreactive axon terminals in the corpora allata were localized between the glandular cells, suggesting that PTTH is released at the inner part of this organ.