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Two‐Dimensional Gel Electrophoretic Analysis of the Cerebella from Neuro‐Pathologically‐Mutant Weaver, Nervous and Staggerer Mice 1
Author(s) -
Asaga Hiroaki,
Inoue Tsutomu,
Mikoshiba Katsuhiko
Publication year - 1990
Publication title -
development, growth and differentiation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.864
H-Index - 66
eISSN - 1440-169X
pISSN - 0012-1592
DOI - 10.1111/j.1440-169x.1990.00223.x
Subject(s) - purkinje cell , cerebellum , biology , mutant , microbiology and biotechnology , central nervous system , gel electrophoresis , silver stain , polyacrylamide gel electrophoresis , nervous system , granule cell , biochemistry , endocrinology , neuroscience , enzyme , gene , dentate gyrus
We have analysed the proteins of the cerebella from mutant and control mice by applying high resolution two‐dimensional polyacrylamide gel electrophoresis. The tissue of each cerebellum and also the pallium cerebri were fractionated into water‐soluble and particulate fractions, and these were used in gel electrophoresis. In order to augment the sensitivity for detection of protein spots, we applied silver staining. We used the cerebella from weaver (granule cell deficient), nervous (Purkinje cell deficient), and staggerer (poor dendritic arborization of Purkinje cells) mutant mice. The present technique revealed at least 700 to 800 protein spots. Among the spots detected we found 12 new significantly‐changed proteins in the cerebella of the mutants. The possible significance of these proteins is discussed.