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A Monoclonal Antibody Specific to Embryonic Trunk‐Lateral Cells of the Ascidian Halocynthia roretzi Stains Coelomic Cells of Juvenile and Adult Basophilic Blood Cells
Author(s) -
Nishide Kazuhiko,
Nishikata Takahito,
Satoh Noriyuki
Publication year - 1989
Publication title -
development, growth and differentiation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.864
H-Index - 66
eISSN - 1440-169X
pISSN - 0012-1592
DOI - 10.1111/j.1440-169x.1989.00595.x
Subject(s) - biology , coelom , basophilic , anatomy , embryo , embryogenesis , embryonic stem cell , monoclonal antibody , microbiology and biotechnology , antibody , pathology , immunology , genetics , medicine , gene
In spite of extensive knowledge on the structure and function of ascidian blood cells, little is known about their embryological origin. In the present investigation, the developmental fate of trunk lateral cells (TLCs) was explored using a specific monoclonal antibody. TLCs comprise a group of undefined embryonic cells of the ascidian Halocynthia roretzi , which arise from the A7.6 blastomeres of a 64‐cell embryo. The antigenicity first appeared at the middle tailbud stage in a pair of TLC‐clusters situated lateral to the brain stem of the bilaterally symmetrical embryo. The position and number of stained cells did not change during later embryogenesis until hatching. After hatching, the stained cells were found in the entire trunk region of the swimming larva. After metamorphosis, cells that expressed the antigen were present within the coelom and within the tunic layer of the juvenile. In addition, the antibody stained adult basophilic blood cells. These observations suggest a relationship of this group of embryonic cells with the prospective blood forming mesenchymal cells.