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Injection of Antibodies into Grasshopper Eggs as a Method for Studying Embryonic Development
Author(s) -
Petrey Dwayne,
Buster Dan,
Donato Katja K.,
Anderson Hilary
Publication year - 1989
Publication title -
development, growth and differentiation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.864
H-Index - 66
eISSN - 1440-169X
pISSN - 0012-1592
DOI - 10.1111/j.1440-169x.1989.00299.x
Subject(s) - embryogenesis , grasshopper , antibody , embryo , embryonic stem cell , insect , biology , andrology , microbiology and biotechnology , immunology , anatomy , ecology , medicine , biochemistry , gene
Procedures are described for injecting antibodies into grasshopper eggs at stages prior to 50% of embryonic development. A microdispenser is used to permit the accurate delivery of small, predetermined volumes of antibody solution. Injected antibodies are taken up by the embryo and reach their appropriate binding sites within 1 hr of injection. Antibody complexes remain stable for at least 24 hr. The method provides a useful tool for investigating developmental processes during early insect embryogenesis.