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Ultrastructural Localization of Calcium in the Acrosome and Jelly Coat of Starfish Gametes
Author(s) -
Sousa Mário,
Azevedo Carlos
Publication year - 1989
Publication title -
development, growth and differentiation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.864
H-Index - 66
eISSN - 1440-169X
pISSN - 0012-1592
DOI - 10.1111/j.1440-169x.1989.00227.x
Subject(s) - spermatozoon , ultrastructure , acrosome , starfish , calcium , coat , oocyte , gamete , microbiology and biotechnology , chemistry , biology , biophysics , anatomy , human fertilization , sperm , botany , embryo , ecology , organic chemistry , paleontology
The potassium pyroantimonate technique was employed to localize calcium ultrastructurally on both male and female starfish gamete regions that first interact at fertilization. In the spermatozoon of Marthasterias glacialis , antimonate precipitates in the peripheral dense component of the acrosomal vesicle, while in the oocyte it precipitates in the jelly coat and beneath the oolemma. Calcium was identified in the precipitates by testing the chelator‐sensitivity and by X‐ray microanalysis of the precipitates.