Premium
Synthesis of Collagen‐Like Proteins in Embryonic Organs of the Sea Urchin, Hemicentrotus pulcherrimus
Author(s) -
Mizoguchi Hazime,
Fujiwara Akiko,
Yasumasu Ikuo
Publication year - 1989
Publication title -
development, growth and differentiation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.864
H-Index - 66
eISSN - 1440-169X
pISSN - 0012-1592
DOI - 10.1111/j.1440-169x.1989.00189.x
Subject(s) - hemicentrotus , spicule , embryo , sea urchin , microbiology and biotechnology , embryogenesis , biology , gastrulation , anatomy , lytechinus variegatus , embryonic stem cell , blastula , chemistry , biochemistry , sponge spicule , gene
In sea urchin embryos exposed to 14 C‐proline at 20°C for 3 hr at the gastrula, prism or pluteus stage, 14 C‐radioactivity was found in hot acid‐extractable proteins, in which more than 4% of the radioactivity was detectable in hydroxyproline residues. In these embryos, 14 C‐radioactivity in collagen‐like proteins was found in the archenteron, spicule and embryo‐wall cells. The rate of synthesis of collagen‐like proteins was highest in the archenteron in the mid‐gastrula stage, in the embryo‐wall cells in the prism stage and in the spicule in the pluteus stage. The rate of synthesis decreased in the archenteron and increased in embryo‐wall cells in the period between the mid‐ and late‐gastrula stages, when the rate of synthesis in the spicule was quite low. Thereafter, the rate decreased slightly in the embryo‐wall cells, was maintained in archenteron and increased markedly in the spicule. The rates of synthesis of collagen‐like proteins are high in these embryonic organs at stages at which development and growth respectively, occur in embryos. Therefore, synthesis of collagen‐like proteins probably supports morphogenesis in these embryonic organs.