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Roles for Potassium and Calcium Channels in the Initiation of Sperm Motility in Rainbow Trout.
Author(s) -
TANIMOTO SATOMI,
MORISAWA MASAAKI
Publication year - 1988
Publication title -
development, growth and differentiation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.864
H-Index - 66
eISSN - 1440-169X
pISSN - 0012-1592
DOI - 10.1111/j.1440-169x.1988.00117.x
Subject(s) - motility , sperm motility , verapamil , sperm , channel blocker , rainbow trout , calcium channel blocker , tetrodotoxin , tetraethylammonium , charybdotoxin , calcium , potassium channel blocker , chemistry , andrology , biology , biophysics , potassium channel , potassium , microbiology and biotechnology , fish <actinopterygii> , fishery , medicine , organic chemistry
It is well known that the motility of spermatozoa in rainbow trout is suppressed by K + . We showed here that although trout sperm are completely immotile in medium containing 5 mM K + , motility was initiated by the subsequent addition of several mM Ca 2+ , suggesting that both K + and Ca 2+ are related to the process of the initiation of sperm motility. It was further found that K + channel blockers tetraethylammonium, nonyltriethylammonium, Ba 2+ and Cs + , as well as the Ca 2+ channel blocker verapamil, inhibited the initiation of sperm motility at doses at which these reagents inhibit chnnel‐related functions in other cells. However, Na + channel blocker, tetrodotoxin and anion channel blocker 4, 4‐diisothiocyatatostilbene‐2, 2′‐disulfonic acid inhibited the motility only at extremely high doses. These results suggest that transport of K + and Ca 2+ through ion channels at the plasma membrane of spermatozoa is the first event that triggers the initiation of sperm motility in rainbow trout.