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Changes in the Level of Histone H4 mRNA in Xenopus leavies Embryogenesis.
Author(s) -
SHIOKAWA KOICHIRO,
ATSUCHI YASUO,
WOODLAND HUGH R.
Publication year - 1987
Publication title -
development, growth and differentiation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.864
H-Index - 66
eISSN - 1440-169X
pISSN - 0012-1592
DOI - 10.1111/j.1440-169x.1987.00571.x
Subject(s) - xenopus , embryogenesis , microbiology and biotechnology , messenger rna , histone , histone h4 , biology , embryo , chemistry , genetics , gene
In Xenopus laevis , the change in the amount of histone H4 mRNA per embryo measured by Northern blotting methods follows a unique change during early embryogenesis: It starts to increase first at the blastula stage, doubles by the gastrula stage then decreases considerably at the neurula stage, and then increases again from the tailbud stage on. The present paper establishes these developmental changes, and furthermore, provides evidence that the synthesis of H4 mRNA starts or at least increases to a detectable level at the midblastula stage as shown by S–1 protection analysis of the expression of paternal histone H4 genes in X. borealis (♀) and X. laevis (♂) hybrid embryos.