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Early Reciprocal Interactions between Ectoderm and Chordamesoderm: Statistical Evaluation of Classical Embryological Experiments
Author(s) -
LEYHAUSEN CLAUDIA
Publication year - 1987
Publication title -
development, growth and differentiation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.864
H-Index - 66
eISSN - 1440-169X
pISSN - 0012-1592
DOI - 10.1111/j.1440-169x.1987.00271.x
Subject(s) - ectoderm , ambystoma mexicanum , biology , embryo , embryogenesis , anatomy , microbiology and biotechnology , regeneration (biology) , axolotl
Embryos of the urodele Ambystoma mexicanum were used in time set experiments for the analysis of ectoderm‐chrodamesoderm interactions in “primary embryonic induction”. The influence of chordamesoderm‐age (CM‐age), ectoderm‐age (CM‐age), ectoderm‐mass (E‐mass) on differentiation processes were investigated. The results revealed that early interactions exist, which, however, seem to be dominated by the ectoderm in various respect: a) the segregations of the CNS is due to the autonomous change in ectoderm competence phases; b) based on the temporal sequence of competence phases, influences are exerted by the ecto‐neuroderm which support the chordamesoderm in its self‐differentiation capability to chorda and other mesodermal tissues. Consequently, reciprocal impulses from these newly differentiated tissues are warranted (feedback to ectoderm); c) co‐ordination of the cranialization effect and corresponding ectodermal competence phase is achieved; d) the inductive efficiency of the chordamesoderm is strongly supported. Three statistical tests (regression, correlation, information analyses) were conducted (dependant variables: quality and quantity of the differentiated structures; independant variables: experimental factors). All three tests equally rendered statistical significance for the following order of decreasing importance the experimental factors play in induction and differentiation processes: E‐age, E‐mass, CM‐age.