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Purification of Mesodermal‐inducing Substances from Carp Swim Bladders I. Extraction and Isoelectric Focusing
Author(s) -
ASASHIMA M.,
NAKANO H.,
MATSUNAGA T.,
SUGIMOTO M.,
TAKANO H.
Publication year - 1987
Publication title -
development, growth and differentiation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.864
H-Index - 66
eISSN - 1440-169X
pISSN - 0012-1592
DOI - 10.1111/j.1440-169x.1987.00221.x
Subject(s) - biology , ectoderm , mesenchyme , gastrulation , embryo , mesoderm , swim bladder , carp , anatomy , microbiology and biotechnology , biochemistry , embryogenesis , fish <actinopterygii> , embryonic stem cell , gene , fishery
Mesodermal‐inducing substances were isolated from the swim bladders of carp ( Carassius auratus ). These substances, extracted with 8 M urea, can induce the development of early gastrula ectoderm cells of newts into mesodermal tissues, such as muscles, notochords, mesenchyme, renal tubules, and so on, to a high degree. Isoelectric focusing was used to isolate them as a first purifying step, and five component parts with different pH ranges were obtained. Each part was tested for mesodermal‐inducing activity. On the basis of the extent and percentage of the induced tissues, two of them (parts C and D with pH ranges 6.0 to 7.0 and 7.0 to 9.0, respectively) were found to have high inducing activity, and the others had lower activity. The substances present in part C showed only low mesodermal‐inducing activity, when diluted with gamma‐globulin 100‐fold. Apparently highly active mesodermal‐inducing substances exist in the two parts C and D.