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Establishment of the Embryo‐derived Stem (ES) Cell Lines from Mouse Blastocysts: Effects of the Feeder Cell Layer.
Author(s) -
SUEMORI HIROFUMI,
NAKATSUJI NORIO
Publication year - 1987
Publication title -
development, growth and differentiation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.864
H-Index - 66
eISSN - 1440-169X
pISSN - 0012-1592
DOI - 10.1111/j.1440-169x.1987.00133.x
Subject(s) - embryonic stem cell , inner cell mass , embryo , cell culture , karyotype , syngenic , biology , microbiology and biotechnology , ploidy , cell , cellular differentiation , germ layer , embryogenesis , blastocyst , genetics , in vitro , chromosome , induced pluripotent stem cell , gene
The ES ceii lines are embryo‐derived stem cell lines directly isolated from the inner cell mass of mouse blastocysts using feeder cell layer. We have established a number of ES cell lines from 129 or C57BL/6 strain mice by using the feeder layer of the STO cells (from ATCC) or the primary embryonic fibroblasts, which was obtained by trypsinizing the 16‐day‐old BALB/c mouse fetus. The ES cell lines established on the STO feeder layer showed differentiation into various tissues in solid tumors when injected into syngenic mice. Karyotype was, however, nearly tetraploid. The ES cell lines established on the primary fibroblasts exhibited differentiation into larger variety of tissues in solid tumors. Karyotype was almost diploid and majority of the cells kept normal set of chromosomes in G‐banding. We conclude that the primary fibroblasts are better feeder layer than the STO cells for establishment and maintenance of the ES cell lines.