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Cell Fusion and Its Application to Genetic Analysis of Development in Dictyostelium discoideum
Author(s) -
SUGIURA KATSUMI,
ISHIKAWA AKIRA
Publication year - 1986
Publication title -
development, growth and differentiation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.864
H-Index - 66
eISSN - 1440-169X
pISSN - 0012-1592
DOI - 10.1111/j.1440-169x.1986.00597.x
Subject(s) - dictyostelium discoideum , cell fusion , fusion , mycetozoa , computational biology , microbiology and biotechnology , biology , cell , genetics , gene , linguistics , philosophy
Dictyostelium cells were fused by a modification of the polyethylene glycol method of Kuhn and Parish. In the modified method Tricine buffer and Concanavalin A were used in place of Ca ++ . The efficiency of genetic complementation through cell fusion was about 10 times higer by the modified method than by the original method with glycine buffer and Ca ++ . Complementation between developmental mutants without any selectable growth character was clearly detected by the modified system, at efficiencies of about 1 in 10–20 surviving cells.