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Accumulation and Distribution of Extracellular Matrix as Revealed by Scanning Electron Microscopy in Freeze‐Dried Newt Embryos Before and During Gastrulation
Author(s) -
KOMAZAKI SHINJI
Publication year - 1986
Publication title -
development, growth and differentiation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.864
H-Index - 66
eISSN - 1440-169X
pISSN - 0012-1592
DOI - 10.1111/j.1440-169x.1986.00285.x
Subject(s) - blastocoel , gastrulation , blastula , extracellular matrix , cleavage (geology) , microbiology and biotechnology , embryo , anatomy , chemistry , polarity in embryogenesis , nanotopography , embryogenesis , biology , fracture (geology) , paleontology
A scanning electron‐microscopic study was carried out on the extracellular matrices (ECMs) in freeze‐dried newt embryos from the cleavage to the gastrula stage. The results revealed the appearance, accumulation and distribution of two types of ECMs, a fibrillar ECM in the blastocoel and an amorphous ECM on the inner surface of the blastocoelic wall (BW). The fibrillar ECM first appeared in the blastocoel at the cleavage stage and increased notably in quantity at the blastula and gastrula stages. On the other hand, the amorphous ECM was initially detected on the inner surface of the BW at the beginning of gastrulation and it increased in quantity during gastrulation. With the progress of archenteron invagination, the amorphous ECM was found to be deposited in the space between the BW and migrating cells.