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Microtubules During Blastoderm Formation in the Egg of the Silkworm, Bombyx mori L.
Author(s) -
TAKESUE SACHIKO,
KEINO HIROOMI
Publication year - 1986
Publication title -
development, growth and differentiation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.864
H-Index - 66
eISSN - 1440-169X
pISSN - 0012-1592
DOI - 10.1111/j.1440-169x.1986.00267.x
Subject(s) - blastoderm , microtubule , cytoplasm , microbiology and biotechnology , nucleus , biology , yolk , mitosis , organelle , astral microtubules , spindle apparatus , cell division , cell , embryogenesis , embryo , genetics , ecology
Microtubules in the silkworm egg, Bombyx mori , were observed by electron microscopy, in order to investigate the relationship between cytoskeletal organelles and the migration of energids, the cleavage nuclei accompanied by the associated cytoplasm, near the egg surface or during blastoderm formation. Numerous microtubules were observed in the associated cytoplasm of an energid even in the interphase of mitosis. At about 8.5 hr after oviposition, when many energids had already cleft and distributed near the peripheral yolk granule region, long microtubules distributed radially from the perinuclear region to the periphery in the associated cytoplasm. When an energid was protruding, the microtubules above the nucleus distributed at a more acute angle than those under the nucleus. When a blastoderm cell had just been formed, the microtubules were observed only under the nucleus. Colchicine, an inhibitor of microtubules, stopped the migration of energids and inhibited the formation of blastoderm cells even after many energids had already distributed at the peripheral yolk granule region. The relationship between the microtubules and the migration of energids near the egg surface or during blastoderm formation was discussed.