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Effects of Low Temperature on Differentiation of Dictyostelium Cells in the Vegetative and Preaggregation Stages
Author(s) -
MAEDA MINEKO
Publication year - 1986
Publication title -
development, growth and differentiation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.864
H-Index - 66
eISSN - 1440-169X
pISSN - 0012-1592
DOI - 10.1111/j.1440-169x.1986.00259.x
Subject(s) - dictyostelium discoideum , spore , antigen , sporogenesis , vacuole , biology , mycetozoa , dictyostelium , microbiology and biotechnology , chemistry , biochemistry , cytoplasm , immunology , gene
The effects of low temperature on differentiation of Dictyostelium discoideum cells in the vegetative and preaggregation stages were examined immunohistochemically and electronmicroscopically. Ultimately cells in both stages were found to stain with FITC‐conjugated anti‐ Dictyostelium mucoroides spore antibody at low tempeature (5°C) in nutrient‐rich medium under submerged conditions. These cells were sensitive to 0.1% Triton X‐100. In contrast, cells in the late aggregation stage were converted to detergent‐resistant spores. Irrespective of their high stainability with antispore‐Ig, these cells did not contain the prespore‐specific vacuoles (PSVs) and spore‐coats characteristic of stainable cells. From these facts, it is concluded that cells in the vegetative and preaggregation stages are unable to differentiate at low temperature into prespore and spore cells, which have PSVs and spore‐coats, respectively. The composition of spore‐specific (SS) antigens accumulated by these cells was examined by immunoblotting. Results showed that under these conditions the cells mainly produced one SS‐antigen, namely SP70. The effects of cell density and components of the medium on the production of SS‐antigens were also examined.