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Developmental Fates of Blastomeres of Eight‐Cell‐Stage Xenopus laevis Embryos
Author(s) -
MASHO RIE,
KUBOTA HIROSHI Y.
Publication year - 1986
Publication title -
development, growth and differentiation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.864
H-Index - 66
eISSN - 1440-169X
pISSN - 0012-1592
DOI - 10.1111/j.1440-169x.1986.00113.x
Subject(s) - blastomere , biology , notochord , embryo , xenopus , anatomy , neural tube , embryogenesis , dorsum , microbiology and biotechnology , genetics , gene
The developmental fates of animal, vegetal, dorsal, and ventral egg regions of Xenopus laevis embryos were examined. For this purpose, a tracer enzyme (horseradish peroxidase) was injected bilaterally into pairs of eight‐cell‐stage blastomeres and the clonal organization of marked cells in the early tail‐bud embryos was examined. The epidermis over most of the body originated from animal‐ventral micromeres, but that in the head originated from animal‐dorsal blastomeres and that in the area surrounding the anus originated from vegetal‐ventral blastomeres. The neural tube originated from animal‐dorsal, vegetal‐dorsal, and animal‐ventral blastomeres. These results were consistent with those of previous studies. But in contrast to previous findings, results showed that the entire notochord is derived from animal‐dorsal micromeres and that the somites originate from all four bilateral pairs of blastomeres in the eight‐cell stage. These results are discussed in relation to the current maps of prospective fates based on results of vital‐dye staining. Morphogenetic movements are also discussed on the basis of the clonal organization demonstrated in the present study.