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Unique Pattern of Gene Expression in the Erythroid Precursor Cells
Author(s) -
MISHINA YUJI,
KATO TAKASHI,
URABE AKIO,
TAKAKU FUMIMARO,
NATORI SHUNJI,
OBINATA MASUO
Publication year - 1986
Publication title -
development, growth and differentiation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.864
H-Index - 66
eISSN - 1440-169X
pISSN - 0012-1592
DOI - 10.1111/j.1440-169x.1986.00001.x
Subject(s) - erythroblast , erythropoietin , biology , percoll , microbiology and biotechnology , gene , gene expression , messenger rna , erythropoiesis , haematopoiesis , in vitro , genetics , stem cell , anemia , medicine
Erythroid cells were fractionated by preformed Percoll density gradient from livers of 12.5 day old mouse fetuses. With combination of lysing of mature erythroid cells, the CFU‐E (colony forming unit of erythroid) was enriched as high as 30% pure. The mRNA levels of the rt‐genes previously cloned as genes expressed in the reticulocytes are estimated in the fractionated erythroid cells. These rt‐genes show a drastic change in expression during erythroid differentiation; Their expression was not detectable at the CFU‐E cell stage. But it reached to maximum at the polychromatic erythroblast (stage I) and then decreases with maturation. The result suggests that mRNA synthesis of these rt‐genes may be induced after the stimulation of erythropoietin.