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Detection of the Initial Step of Mesenchymal Differentiation of Teratocarcinoma Cells Using the Monoclonal Antibody Eccd‐1 1
Author(s) -
YOSHIDANORO CHIKAKO,
TAKEICHI MASATOSHI,
OKADA T. S.
Publication year - 1985
Publication title -
development, growth and differentiation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.864
H-Index - 66
eISSN - 1440-169X
pISSN - 0012-1592
DOI - 10.1111/j.1440-169x.1985.00673.x
Subject(s) - teratocarcinoma , monoclonal antibody , mesenchymal stem cell , microbiology and biotechnology , chemistry , antibody , biology , cellular differentiation , immunology , biochemistry , gene
The monoclonal antibody ECCD‐1 recognizes the Ca 2+ ‐dependent cell‐cell adhesion molecule of teratocarcinoma stem cells (EC cells) and of a certain class of differentiated epithelial cells. It actively disrupts cell‐cell adhesion when added to monolayer cultures of these cells, but does not affect adhesion of mesenchymal or neuronal cells. When ECCD‐1 was added to clonal cultures of EC cells (PCC3/A/1 line), all the cells were initially sensitive to the antibody, but after 5 to 6 days of culture a fraction of the cells in certain colonies no longer reacted with the antibody although they expressed alkaline phosphatase activity, which is a marker of undifferentiated EC cells. We isolated these ECCD‐1‐resistant cells by recloning and examined their differentiation by clonal culture. Most of them differentiated into fibroblastic cells and a few into skeletal muscle‐like cells, but none differentiated into any other cell types. From these observations, we suppose that the ECCD‐1‐resistant population of EC cells are committed to mesenchymal differentiation. The use of ECCD‐1, thus, permitted us to detect EC cells at the initial stage of a particular differentiation pathway.