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Prestalk/prespore Differentiation of Dictyostelium Cells under the Conditions Favoring Stalk or Spore Cell Formation *
Author(s) -
NAKAHARA YASUO,
NOCE TOSHIAKI,
TAKEUCHI IKUO
Publication year - 1985
Publication title -
development, growth and differentiation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.864
H-Index - 66
eISSN - 1440-169X
pISSN - 0012-1592
DOI - 10.1111/j.1440-169x.1985.00591.x
Subject(s) - dictyostelium discoideum , stalk , mycetozoa , biology , polyclonal antibodies , antibody , monoclonal antibody , microbiology and biotechnology , spore , cellular differentiation , cell , antigen , dictyostelium , immunology , gene , biochemistry , horticulture
The differentiation processes of Dictyostelium discoideum cells under the conditions which favored either stalk or spore cell formation were examined by the use of prestalk‐ and prespore‐specific antibodies. In stalk cell‐forming conditions, cells reactive with prestalk‐specific monoclonal antibody (C1) increased rapidly early in development and later differentiated into stalk cells. No or only a few cells became reactive with prespore‐specific monoclonal (B6) and polyclonal (antispore) antibodies. Despite the fact that most cells terminally became spores under spore cell‐forming conditions, cells were first stained with the C1 antibody before becoming reactive with the B6 antibody. Unlike the case of normal development where cells coincidentally become reactive with the B6 and antispore antibodies, the appearance of the cells reactive with the latter was either delayed or suppressed. In conclusion, under either spore or stalk cell‐forming conditions, the appearance of the prestalk antigen preceded that of the prespore one, which is consistent with normal development.