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One Millimeter large Oocytes as a Tool to Study Hormonal Control of Meiotic Maturation in Starfish: Role of the Nucleus in Hormone‐stimulated Phosphorylation of Cytoplasmic Proteins
Author(s) -
PICARD ANDRÉ,
PEAUCELLIER GÉRARD,
BOUFFANT FRANCOISE,
DOREE MARCEL
Publication year - 1985
Publication title -
development, growth and differentiation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.864
H-Index - 66
eISSN - 1440-169X
pISSN - 0012-1592
DOI - 10.1111/j.1440-169x.1985.00251.x
Subject(s) - germinal vesicle , phosphorylation , cytoplasm , biology , microbiology and biotechnology , oocyte , phosphoprotein , xenopus , nucleus , starfish , meiosis , protein phosphorylation , microinjection , protein kinase a , biochemistry , embryo , ecology , gene
Single nuclei (germinal vesicles) manually isolated from large oocytes of the starfish Echinaster sepositus , as well as the complementary anucleated oocytes, were used to investigate the early changes of protein phosphorylation which occur from 1‐MeAde addition to germinal vesicle breakdown (GVBD). Stimulation of protein phosphorylation was already evident in the nucleus shortly after 1‐MeAde addition (18 min, thus about 0.40x the time required for GVBD), although it began first in the cytoplasm. No translocation of phosphoprotein across the nuclear envelope was detected before GVBD. Presence of the nucleus is not required for the hormone to stimulate protein phosphorylation in the remaining part of the oocytetin:fact the patterns of protein phosphorylation in enucleated oocytes were found to be identical, whether enucleation was performed after or before hormonal treatment. Cytoplasm taken at the time of GVBD from maturing Echinaster oocytes induces meiotic maturation when transferred in stage VI immature oocytes of the amphibian Xenopus laevis.