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Establishment of the Neural Differentiation Pattern in the Prospective Prosencephalic Ectoderm of the Chick Embryo ( Gallus domesticus )
Author(s) -
KHARE M. K.,
CHOUDHURY S.
Publication year - 1985
Publication title -
development, growth and differentiation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.864
H-Index - 66
eISSN - 1440-169X
pISSN - 0012-1592
DOI - 10.1111/j.1440-169x.1985.00083.x
Subject(s) - ectoderm , biology , anatomy , embryo , endoderm , embryogenesis , microbiology and biotechnology , cellular differentiation , genetics , gene
Small graft pieces (size: 0.15 × 0.2 mm to 0.1 × 0.4 mm and 0.25 × 0.25 mm) were isolated from the prospective prosencephalic ectoderm of the definitive primitive streak (st. 4) and head process (st. 5) chick blastoderms, and cultured in vivo intracoelomically. In all 1437 graft pieces isolated from 448 donor blastoderms were transplanted into the coelom of 941 host embryos; 216 hosts died before reaching 12 days. The 725 surviving hosts, which carried 1241 implants, yielded 304 analysable grafts. The very small size of the isolates affected the recovery rate. The histological analysis of the recovered grafts reveals that the frequency of neuralization was higher in the grafts isolated from the central region of the prospective prosencephalic ectoderm than in those from its peripheral region. A centrifugal extension of differentiation tendencies of prosencephalic structures was observed from st. 4 to st. 5. At st. 5 frequency of telencephalic structures was high in the grafts of the anterior region, whereas that of diencephalic structures was high in those of the more posterior region. Eye structures (retina as well as tapetum) differentiated in an area measuring ca. 0.4 × 0.1 mm extending transversely about 0.1 mm anterior to the head process at st. 5. Structural elements of the lens were not observed in any graft.