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Inhibitory Effect of Calmodulin Antagonists and Calcium Antagonists on Fertilization‐Induced Cyanide‐Insensitive Respiration in Sea Urchin Eggs
Author(s) -
TSUCHIDA EMI,
FUJIWARA AKIKO,
FUJINO YUKIO,
YONAGA TAKARA,
YASUMASU IKUO
Publication year - 1985
Publication title -
development, growth and differentiation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.864
H-Index - 66
eISSN - 1440-169X
pISSN - 0012-1592
DOI - 10.1111/j.1440-169x.1985.00063.x
Subject(s) - sea urchin , human fertilization , calcium , calmodulin , respiration , chemistry , biology , inhibitory postsynaptic potential , botany , ecology , endocrinology , anatomy , organic chemistry
During initial several minutes after fertilization, sea urchin eggs exhibited high rate of respiration which was only slightly inhibited by cyanide. This cyanide‐insensitive respiration was inhibited by calcium antagonists, diltiazem and verapamil, and calmodulin antagonists, N‐(6‐aminohexyl)‐5‐chloro‐1‐naphthalensulfonamide hydrochloride (W‐7), N‐(6‐aminohexyl)‐1‐naphthalenesulfonamide hydrochloride (W‐5) and chlorpromazine, which were added within 1 min after insemination. The inhibitory effect of W‐7 on cyanide‐insensitive respiration was higher than that of W‐5. Cyanide‐sensitive respiration of fertilized eggs observed after this initial period was not inhibited by these compounds. Ca 2+ influx in eggs just after fertilization was inhibited by calcium antagonists but was rather enhanced by calmodulin antagonists. Fertilization‐induced stimulation of cyanide‐insensitive respiration probably results from calmodulin‐dependent reactions which are activated by Ca 2+ influx.