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Scanning Electron Microscopy of the Extracellular Matrix of Amphibian Gastrulae by Freeze‐drying
Author(s) -
KOMAZAKI SHINJI
Publication year - 1985
Publication title -
development, growth and differentiation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.864
H-Index - 66
eISSN - 1440-169X
pISSN - 0012-1592
DOI - 10.1111/j.1440-169x.1985.00057.x
Subject(s) - extracellular matrix , scanning electron microscope , anatomy , matrix (chemical analysis) , ultrastructure , chemistry , blastocoel , biophysics , electron microscope , amphibian , materials science , biology , microbiology and biotechnology , embryo , gastrulation , embryogenesis , composite material , optics , physics , ecology , chromatography
In order to demonstrate the extracellular matrix (ECM) which tends to be missing from specimens prepared by ordinary procedures, a freeze‐drying method was applied to gastrulae of the newt ( Cynops pyrrhogaster ) without prefixation and the embryos were examined with a scanning electron microscope (SEM). It was revealed that a prominent amount of fibrillar ECM occupied the blastocoel and amorphous ECM covered the inner surface of the blastocoelic wall (BW) as well as the surface of migrating cells. These results were compared with those obtained from observations of specimens fixed chemically.