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Intracellular Localization and Sedimentation Coefficient of DNA Ligase in Oocytes of the Starfish, Asterina pectinifera
Author(s) -
OISHI NOBORU,
SHIMADA HIRAKU
Publication year - 1984
Publication title -
development, growth and differentiation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.864
H-Index - 66
eISSN - 1440-169X
pISSN - 0012-1592
DOI - 10.1111/j.1440-169x.1984.00571.x
Subject(s) - germinal vesicle , biology , oocyte , cytoplast , strongylocentrotus droebachiensis , polar body , human fertilization , cytochalasin b , starfish , sedimentation coefficient , embryo , microbiology and biotechnology , botany , anatomy , genetics , biochemistry , sea urchin , cell , ecology , enzyme
When immature oocytes of Asterina pectinifera were separated into karyoplasts (nuclear fragments) and cytoplasts (anuclear fragments) by cytochalasin B treatment and centrifugation in a sucrose gradient, almost all their DNA ligase activity was recovered in the karyoplasts. Thus, DNA ligase seems to be localized in the germinal vesicles or their vicinity in starfish oocytes. No ontogenic change in the activity of DNA ligase per oocyte, egg or embryo, or in its sedimentation coefficient (4.1 S) was observed during oocyte maturation, fertilization, or early development.