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Protein Synthetic Activities during Spermiogenesis in the Sea Urchin: An high Resolution Autoradiographic Study of 3 H‐Leucine Incorporation
Author(s) -
NICOTRA ANTONIETTA,
ARIZZI MARIO,
GALLO PATRIZIA VALENTINA
Publication year - 1984
Publication title -
development, growth and differentiation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.864
H-Index - 66
eISSN - 1440-169X
pISSN - 0012-1592
DOI - 10.1111/j.1440-169x.1984.00273.x
Subject(s) - spermatid , spermiogenesis , sea urchin , cytosol , biology , leucine , paracentrotus lividus , nucleus , biochemistry , mitochondrion , microbiology and biotechnology , chemistry , biophysics , botany , enzyme , amino acid , sperm
Protein synthetic activity has been studied during spermiogenesis of Paracentrotus lividus by high‐resolution autoradiography using 3 H‐leucine as a labeled precursor. Under the adopted experimental conditions 3 H‐leucine is incorporated during the whole spermiogenesis period. The early spermatid is the most active stage and it shows labeling over the nucleus, the cytosol and the mitochondria. Nuclear 3 H‐leucine incorporation progressively decreases as spermiogenesis proceeds. Cytosol labeling shows similar values at early and intermediate spermatid and it undergoes a considerable decreases at late spermatid. Mitochondrial grain density increases from early to intermediate spermatid and it remains almost constant at late spermatid. Our results are compared with the data reported for other animal groups and possible functions of the observed protein synthesis are discussed.