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Appearance of Extrachromosomal Circular DNA in Lymphocytes from Developing Chick Bursa
Author(s) -
TODA MASAAKI,
YAMAGISHI HIDEO
Publication year - 1984
Publication title -
development, growth and differentiation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.864
H-Index - 66
eISSN - 1440-169X
pISSN - 0012-1592
DOI - 10.1111/j.1440-169x.1984.00197.x
Subject(s) - extrachromosomal dna , bursa of fabricius , biology , dna , microbiology and biotechnology , embryo , embryonic stem cell , haematopoiesis , zygote , virology , genetics , embryogenesis , plasmid , stem cell , gene , virus
Lymphocytes were purified from the developing bursa of Fabricius of chick embryos and chickens and pressed with a mica sheet. Then the extruded DNA complexes were adsorbed to the mica and processed for electron microscopy. Circular DNA complexes were found at 40 to 60 copies in all bursal lymphocyte except 12‐day‐old embryonic bursas, which contained 150 copies. Circular DNA complexes of more than 1 μm (larger circular DNA) appeared in 12‐day‐old embryonic bursas at more than 20 copies per cell, and subsequently decreased in number to less than 10 copies per cell. These changes in the size distribution and copy number of circular DNA coincided with lymphocyte differentiation of the hemopoietic precursor cells in the bursa after seeding.